Western blot analysis of SIM1 using SIM1 Polyclonal Antibody (Product # PA5-37887) (1 µg/mL) in staining of HEK293 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot of SIM1 in HEK293 lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a SIM1 polyclonal antibody (Product # PA5-37887) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supplier Page from Thermo Fisher Scientific for SIM1 Antibody